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pPS2986
(Plasmid #8928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-PK
  • Backbone manufacturer
    Warner Greene
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IN
  • Alt name
    pEGFP-PK-IN
  • Alt name
    HIV-1 integrase
  • Species
    HIV
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • PK (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generates pEGFP-PK-codon optimized IN fusion. Localizes to cytoplasm.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2986 was a gift from Pamela Silver (Addgene plasmid # 8928 ; http://n2t.net/addgene:8928 ; RRID:Addgene_8928)

  • For your References section:

    Intracellular transport of human immunodeficiency virus type 1 integrase. Devroe E, Engelman A, Silver PA. J Cell Sci. 2003 Nov 1. 116(Pt 21):4401-8. 10.1242/jcs.00747 PubMed 13130095
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