pPS2977
(Plasmid #8927)

• Depositing Lab: Pamela Silver
• Publication: Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-51.

Backbone

• Vector backbone: pECMV-FLAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NDR1
• Alt name: pFLAG-NDR1
• Species: H. sapiens (human)
• Mutation: see comments
• Entrez Gene: STK38 (a.k.a. NDR, NDR1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NDR1 ORF PCR amplified from Hela cDNA.

There is an E102G mutation in the NDR1. The effect of this mutation is unknown, but this was the construct used in the publication.