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Addgene

pTX176
(Plasmid #89260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTX171
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

p8xloxA_mini35S::Cas9::RZ cleavage site::gRNA::RZ cleavage site::RZ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTX176 was a gift from Daniel Voytas (Addgene plasmid # 89260 ; http://n2t.net/addgene:89260 ; RRID:Addgene_89260)

  • For your References section:

    A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Tang X, Zheng X, Qi Y, Zhang D, Cheng Y, Tang A, Voytas DF, Zhang Y. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. 10.1016/j.molp.2016.05.001 PubMed 27212389
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