pPS2971
(Plasmid
#8926)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8926 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS/U6
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNDR1
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Alt namepBS/U6-NDR
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Alt namesiRNA NDR1
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SpeciesH. sapiens (human)
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GenBank IDNM_007271
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Entrez GeneSTK38 (a.k.a. NDR, NDR1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
siRNA targeting NDR1. Anneal NDR oligos and ligate into pBS/U6 (U6 promoter cloned into Bluescript). Small hairpin RNA corresponds to nucleotides 515-535 of NDR ORF (nucleotides 801-821 of NDR mRNA sequence, Genbank NM_007271). shRNA: 5'-GGGACATGATGACCTTGTTGA-3'.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS2971 was a gift from Pamela Silver (Addgene plasmid # 8926 ; http://n2t.net/addgene:8926 ; RRID:Addgene_8926) -
For your References section:
Retrovirus-delivered siRNA. Devroe E, Silver PA. BMC Biotechnol. 2002 Aug 28. 2():15. 10.1186/1472-6750-2-15 PubMed 12199908