pPS2966
(Plasmid #8925)

• Depositing Lab: Pamela Silver
• Publication: Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-51.

Backbone

• Vector backbone: pGEX-4T1
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NDR1
• Alt name: pGST-NDR1
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Entrez Gene: STK38 (a.k.a. NDR, NDR1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: pGEX5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NDR1 ORF PCR amplified from Hela cDNA. ~1.5 kb insert digested with BamHI/ScaI and inserted into pGEX-4T1 digested with BamHI/SmaI. Completely sequenced and includes the entire NDR ORF as reported in NCBI database. Note: GST-NDR1 is extremely insoluble when expressed in E. coli, possibility that co-expression of Mob2 would help solubilize NDR1.