pAcrF3
(Plasmid
#89246)
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PurposePlasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15TV-L
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAcrF3
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GenBank ID5GNF_A
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJoseph Bondy-Denomy (University of California San Francisco) and Alan Davidson (University of Toronto)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcrF3 was a gift from Blake Wiedenheft (Addgene plasmid # 89246 ; http://n2t.net/addgene:89246 ; RRID:Addgene_89246) -
For your References section:
Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity. Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B. Proc Natl Acad Sci U S A. 2017 Apr 24. pii: 201616395. doi: 10.1073/pnas.1616395114. 10.1073/pnas.1616395114 PubMed 28438998
