pcNB2
(Plasmid
#89242)
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PurposeResurfaced Cell-penetrating 5F7 nanobody
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETduet
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5420
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepcNB2 (5F7)
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Alt namenanobody against HER2
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Insert Size (bp)375
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcNB2 was a gift from Brian McNaughton (Addgene plasmid # 89242 ; http://n2t.net/addgene:89242 ; RRID:Addgene_89242) -
For your References section:
Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery. Bruce VJ, Lopez-Islas M, McNaughton BR. Protein Sci. 2016 Jun;25(6):1129-37. doi: 10.1002/pro.2926. Epub 2016 Apr 4. 10.1002/pro.2926 PubMed 26991318