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Addgene

pcNB1
(Plasmid #89241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pcNB1 (GFP)
  • Alt name
    resurfaced cell-penetrating nanobody against GFP
  • Insert Size (bp)
    378
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcNB1 was a gift from Brian McNaughton (Addgene plasmid # 89241 ; http://n2t.net/addgene:89241 ; RRID:Addgene_89241)

  • For your References section:

    Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery. Bruce VJ, Lopez-Islas M, McNaughton BR. Protein Sci. 2016 Jun;25(6):1129-37. doi: 10.1002/pro.2926. Epub 2016 Apr 4. 10.1002/pro.2926 PubMed 26991318
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