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Addgene

pPS2700
(Plasmid #8924)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNOPMYCA1L
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YRA1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1500
  • Entrez Gene
    YRA1 (a.k.a. YDR381W, SHE11)
  • Tag / Fusion Protein
    • 3x Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer Myc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone from K.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2700 was a gift from Pamela Silver (Addgene plasmid # 8924 ; http://n2t.net/addgene:8924 ; RRID:Addgene_8924)

  • For your References section:

    Messenger RNAs are recruited for nuclear export during transcription. Lei EP, Krebber H, Silver PA. Genes Dev. 2001 Jul 15. 15(14):1771-82. 10.1101/gad.892401 PubMed 11459827
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