pMBPCas2/3
(Plasmid
#89238)
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PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbone2S
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas2/3
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SpeciesPseudomonas aeruginosa PA14
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Tag
/ Fusion Protein
- Strep II-MBP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBPCas2/3 was a gift from Blake Wiedenheft (Addgene plasmid # 89238 ; http://n2t.net/addgene:89238 ; RRID:Addgene_89238) -
For your References section:
Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity. Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B. Proc Natl Acad Sci U S A. 2017 Apr 24. pii: 201616395. doi: 10.1073/pnas.1616395114. 10.1073/pnas.1616395114 PubMed 28438998
Map uploaded by the depositor.
