pAcrF2
(Plasmid #89234)

• Purpose: Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site
• Depositing Lab: Blake Wiedenheft
• Publication: Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012.

Backbone

• Vector backbone: p15TV-L

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AcrF2
• Alt name: Gene 30 from bacteriophage D3112
• GenBank ID: UniProt: Q6TM72
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • AcrF2.gb
• A portion of this plasmid was derived from a plasmid made by: Joseph Bondy-Denomy (University of California San Francisco ) and Alan Davidson (University of Toronto)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry