pAcrF1
(Plasmid
#89233)
-
PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonep15TV-L
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameAcrF1
-
Alt nameGene 35 from bacteriophage JBD30
-
Tags
/ Fusion Proteins
- 6xHis-TEV (N terminal on insert)
- FLAG (N terminal on insert)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byJoseph Bondy-Denomy (University of California San Francisco) and Alan Davidson (University of Toronto)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAcrF1 was a gift from Blake Wiedenheft (Addgene plasmid # 89233 ; http://n2t.net/addgene:89233 ; RRID:Addgene_89233) -
For your References section:
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex. Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B. Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012. 10.1016/j.cell.2017.03.012 PubMed 28340349
Map uploaded by the depositor.
