pCsy_complex
(Plasmid #89232)

• Purpose: Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
• Depositing Lab: Blake Wiedenheft
• Publication: Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012.

Backbone

• Vector backbone: 2D

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Csy1
• Alt name: Cas8f
• Species: Pseudomonas aeruginosa PA14
• GenBank ID: WP_003139224.1

Gene/Insert 2

• Gene/Insert name: Csy2
• Alt name: Cas5f
• Species: Pseudomonas aeruginosa PA14
• GenBank ID: WP_003116910.1

Gene/Insert 3

• Gene/Insert name: Csy3
• Alt name: Cas7f
• GenBank ID: WP_003139222.1
• Tag / Fusion Protein:
  • 6xHis-TEV protease (N terminal on insert)

Gene/Insert 4

• Gene/Insert name: Csy4
• Alt name: Cas6f

Resource Information

• Supplemental Documents:
  • pCsy_complex.gb
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry