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Addgene

pDsred monomer hygro C1- MLPH
(Plasmid #89231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed-Monomer-Hyg-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Melanophilin
  • Species
    H. sapiens (human)
  • Mutation
    wild type
  • GenBank ID
    BC001653.2
  • Entrez Gene
    MLPH (a.k.a. SLAC2-A)
  • Promoter CMV
  • Tag / Fusion Protein
    • Dsred monomer (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer 5'-AGCTGGACATCACCAACCACAACG-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDsred monomer hygro C1- MLPH was a gift from William Gahl (Addgene plasmid # 89231 ; http://n2t.net/addgene:89231 ; RRID:Addgene_89231)
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