pPS2192
(Plasmid #8923)

• Depositing Lab: Pamela Silver
• Publication: Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

Backbone

• Vector backbone: pPS1304
• Backbone size w/o insert (bp): 5200
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Clb2-deltaNLS-GFP
• Alt name: Clb2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1400
• Mutation: Deletion of CLB2 bipartite NLS (aa 183-200), KpnI site introduced at site of NLS deletion
• Entrez Gene: CLB2 (a.k.a. YPR119W)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to pPS2189, but with deletion of NLS. Created by PCR amplifying the fragments of CLB2 ORF upstream (0.5kb) and downstream (0.9kb) of the bipartite NLS and joining them using KpnI ends. The KpnI site introduces a glycine and a threonine in place of the bipartite NLS. 2 micron plasmid.