pPS1597
(Plasmid
#8920)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS808
- Backbone size w/o insert (bp) 5200
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTIF51A
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Alt nameHYP2
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Alt namepGal.GFP.NPL2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneHYP2 (a.k.a. YEL034W, TIF51A)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TIF51A cloned by PCR from genomic DNA (PSY580). GAL1 promoter, 2 micron plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS1597 was a gift from Pamela Silver (Addgene plasmid # 8920 ; http://n2t.net/addgene:8920 ; RRID:Addgene_8920) -
For your References section:
Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling. Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE. Genetics. 2002 Feb . 160(2):393-405. PubMed 11861547