pHES874
(Plasmid
#89196)
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PurposeExpresses mCherry under control of synthetic promoter containing Zif268 binding sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNH604
- Backbone size w/o insert (bp) 6559
- Total vector size (bp) 7957
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepZ-mCherry
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SpeciesS. cerevisiae (budding yeast), Synthetic
- Promoter pZ
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PspOMI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer AACTAATCAGCGGTACCGGG
- 3′ sequencing primer CGCACTCACGTAAACACTTAATC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHES874 was a gift from Hana El-Samad (Addgene plasmid # 89196 ; http://n2t.net/addgene:89196 ; RRID:Addgene_89196) -
For your References section:
Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast. Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H. ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251. 10.1021/acssynbio.6b00251 PubMed 27930885
Map uploaded by the depositor.
