Skip to main content
Addgene

pPS1523
(Plasmid #8919)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPS846
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YRB2
  • Alt name
    GFP-YRB2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    YRB2 (a.k.a. YIL063C)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP YRB2 KpnI-BamHI (5' YRB2 promoter)/BglII-ClaI (GFP-YRB2) was inserted into Kpn/ClaI of pPS846 (CEN URA3 YRB2).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1523 was a gift from Pamela Silver (Addgene plasmid # 8919 ; http://n2t.net/addgene:8919 ; RRID:Addgene_8919)

  • For your References section:

    A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Taura T, Krebber H, Silver PA. Proc Natl Acad Sci U S A 1998 Jun 23;95(13):7427-32. 10.1073/pnas.95.13.7427 PubMed 9636166
Related items: