pMaRSC
(Plasmid #89189)

• Purpose: The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.
• Depositing Lab: David Tirrell
• Publication: Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25.

Backbone

• Vector backbone: pcDNA 3.1
• Backbone size w/o insert (bp): 5519
• Total vector size (bp): 9047
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flag-L274G-T2A-mCherry
• Species: M. musculus (mouse)
• Insert Size (bp): 3528
• GenBank ID: NM_001003913.2
• Entrez Gene: Mars1 (a.k.a. Mars, Metrs, Mtrns)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Alborz Mahdavi (California Institute of Technology)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry