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pPS1358
(Plasmid #8918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPS810
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HRP1
  • Alt name
    pCGF-1C HRP1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HRP1 (a.k.a. YOL123W, NAB4, NAB5)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAL1 promoter, 2 micron plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1358 was a gift from Pamela Silver (Addgene plasmid # 8918 ; http://n2t.net/addgene:8918 ; RRID:Addgene_8918)

  • For your References section:

    Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast. Kessler MM, Henry MF, Shen E, Zhao J, Gross S, Silver PA, Moore CL. Genes Dev. 1997 Oct 1. 11(19):2545-56. 10.1101/gad.11.19.2545 PubMed 9334319
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