pYUB28b-MCS-optimized
(Plasmid #89179)

• Purpose: (Empty Backbone) mycobacteria shuttle vector - pYUB28b with a single change to the MCS
• Depositing Labs: Ted Baker, Ghader Bashiri
• Publication: Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Backbone

• Vector backbone: pYUB28b Addgene #37277
• Backbone manufacturer: Ghader Bashiri
• Backbone size (bp): 4920
• Modifications to backbone: a single change to the MCS (NcoI to SphI which increases its functionality)
• Vector type: Bacterial Expression ; mycobacteria shuttle vector
• Promoter: T7
• Tags / Fusion Proteins:
  • His-tag
  • His-tag

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.

This plasmid is identical to pYUB28b Plasmid #37277, except that a single change was introduced to the MCS (NcoI in the original plasmid is replaced by SphI in this version of the plasmid).