pALPS-ZsGreen-miR-146a
(Plasmid #89174)

• Purpose: encodes human/murine miR-146a and ZsGreen1
• Depositing Lab: Hilary Sheppard
• Publication: pAPM-mmu-miR146a (unpublished)

Backbone

• Vector backbone: pALPS-Puro aka pAPM
• Backbone manufacturer: Silvia Monticelli, Institute for Research in Biomedicine, Bellinzona, Switzerland
• Modifications to backbone: Puromycin cassette was removed and replaced with ZsGreen1
• Vector type: Lentiviral
• Selectable markers: ZsGreen1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miR-146a
• Species: H. sapiens (human), M. musculus (mouse)
• Entrez Gene: MIR146A (a.k.a. MIRN146, MIRN146A, miR-146a, miRNA146A)
• Entrez Gene: Mir146 (a.k.a. Mirn146, miR-146a, mmu-mir-146)
• Promoter: SFFVp

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pALPS-Puro-mu miR-146a (pAPM)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry