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pTYB11-PTBP1-RRM2CCtoSS
(Plasmid #89154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB11
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 7412
  • Total vector size (bp) 7800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S
  • Alt name
    PTBP1 RNA Binding Domain 2 mutant C250S, C251S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    420
  • Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
  • Entrez Gene
    PTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
  • Promoter T7
  • Tags / Fusion Proteins
    • chitin binding domain (N terminal on backbone)
    • Intein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Christophe Maris

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB11-PTBP1-RRM2CCtoSS was a gift from Frédéric Allain (Addgene plasmid # 89154 ; http://n2t.net/addgene:89154 ; RRID:Addgene_89154)

  • For your References section:

    Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS. Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A, Aebersold R, Allain FH. Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235. 10.1038/nmeth.4235 PubMed 28346450
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