pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
(Plasmid #89150)

• Purpose: An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.
• Depositing Lab: Brandon Harvey
• Publication: Gomez et al Science. 2017 Aug 4;357(6350):503-507. doi: 10.1126/science.aan2475.

Backbone

• Vector backbone: pAAV EF1a DIO
• Backbone manufacturer: Karl Deisseroth
• Backbone size w/o insert (bp): 5239
• Modifications to backbone: lox sites were removed when CDS was added between BamHI and EcoRV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HA-tagged hM4D(Gi)
• Alt name: Gq-coupled inhibitory DREADD
• Promoter: EF1a
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: EF1a F1
• 3′ sequencing primer: WPRE R1

Resource Information

• Supplemental Documents:
  • pOTTC1329 - pAAV EF1a HA-hM4D(Gi).ape
• A portion of this plasmid was derived from a plasmid made by: the ORF for hM4D(Gi) was provided by Bryan Roth.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry