pSG5-MAGE-TA360
(Plasmid
#89141)
-
Purposemammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 89141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSG5
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMAGE-A11
-
SpeciesH. sapiens (human)
-
MutationT360A
-
Entrez GeneMAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSG5-MAGE-TA360 was a gift from Elizabeth Wilson (Addgene plasmid # 89141 ; http://n2t.net/addgene:89141 ; RRID:Addgene_89141) -
For your References section:
Epidermal-growth-factor-dependent phosphorylation and ubiquitinylation of MAGE-11 regulates its interaction with the androgen receptor. Bai S, Wilson EM. Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. Epub 2008 Jan 22. 10.1128/MCB.01672-07 PubMed 18212060