VPCT-SUV39H2-L
(Plasmid
#89137)
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Purposemammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to VP16 activation domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVP16-CT
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSUV39H2
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Entrez GeneSUV39H2 (a.k.a. KMT1B)
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Tag
/ Fusion Protein
- VP16 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VPCT-SUV39H2-L was a gift from Elizabeth Wilson (Addgene plasmid # 89137 ; http://n2t.net/addgene:89137 ; RRID:Addgene_89137) -
For your References section:
Androgen receptor regulation by histone methyltransferase Suppressor of variegation 3-9 homolog 2 and Melanoma antigen-A11. Askew EB, Bai S, Parris AB, Minges JT, Wilson EM. Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 10.1016/j.mce.2016.12.028. Epub 2016 Dec 29. 10.1016/j.mce.2016.12.028 PubMed 28042025