pSG5HA-MAGE-2-429
(Plasmid #89133)

• Purpose: mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429
• Depositing Lab: Elizabeth Wilson
• Publication: Askew et al J Biol Chem. 2009 Dec 11;284(50):34793-808. doi: 10.1074/jbc.M109.065979. Epub 2009 Oct 14.

Backbone

• Vector backbone: pSG5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAGE-A11
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: contains aa 2-429
• Entrez Gene: MAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
• Promoter: SV40
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry