GAL-FKBP51
(Plasmid
#89132)
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Purposemammalian expression of FKBP5 also referred to as FKBP51 with N-terminal GAL4 DNA binding domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGALO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFKBP5
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Alt nameFKBP51
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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Entrez GeneFKBP5 (a.k.a. AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10)
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Tag
/ Fusion Protein
- GAL4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL-FKBP51 was a gift from Elizabeth Wilson (Addgene plasmid # 89132 ; http://n2t.net/addgene:89132 ; RRID:Addgene_89132) -
For your References section:
Primate-specific melanoma antigen-A11 regulates isoform-specific human progesterone receptor-B transactivation. Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, Wilson EM. J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 10.1074/jbc.M112.372797. Epub 2012 Aug 13. 10.1074/jbc.M112.372797 PubMed 22891251