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Addgene

pSG5HA-MAGE-112-429
(Plasmid #89131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAGE-A11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    contains aa 112-429
  • Entrez Gene
    MAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
  • Promoter SV40
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5HA-MAGE-112-429 was a gift from Elizabeth Wilson (Addgene plasmid # 89131 ; http://n2t.net/addgene:89131 ; RRID:Addgene_89131)

  • For your References section:

    Epidermal-growth-factor-dependent phosphorylation and ubiquitinylation of MAGE-11 regulates its interaction with the androgen receptor. Bai S, Wilson EM. Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. Epub 2008 Jan 22. 10.1128/MCB.01672-07 PubMed 18212060
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