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Addgene

pPS1323
(Plasmid #8913)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HRB1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2300
  • Entrez Gene
    HRB1 (a.k.a. TOM34)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BalI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GST-HRB1 was excised from pPS1320 and ligated into pGEX-4T-1. See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1323 was a gift from Pamela Silver (Addgene plasmid # 8913 ; http://n2t.net/addgene:8913 ; RRID:Addgene_8913)

  • For your References section:

    Arginine methylation facilitates the nuclear export of hnRNP proteins. Shen EC, Henry MF, Weiss VH, Valentini SR, Silver PA, Lee MS. Genes Dev 1998 Mar 1;12(5):679-91. 10.1101/gad.12.5.679 PubMed 9499403
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