GST-MAGE-2-429
(Plasmid #89129)

• Purpose: bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites
• Depositing Lab: Elizabeth Wilson
• Publication: Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 10.1016/j.mce.2016.12.028. Epub 2016 Dec 29.

Backbone

• Vector backbone: pGEX-4T-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAGE-A11
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Entrez Gene: MAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry