Skip to main content
Addgene

FLAG-MAGE-112-429
(Plasmid #89128)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAGE-A11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    contains aa 112-429
  • Entrez Gene
    MAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-MAGE-112-429 was a gift from Elizabeth Wilson (Addgene plasmid # 89128 ; http://n2t.net/addgene:89128 ; RRID:Addgene_89128)

  • For your References section:

    Melanoma antigen-A11 regulates substrate-specificity of Skp2-mediated protein degradation. Su S, Chen X, Geng J, Minges JT, Grossman G, Wilson EM. Mol Cell Endocrinol. 2017 Jan 5;439:1-9. doi: 10.1016/j.mce.2016.10.006. Epub 2016 Oct 6. 10.1016/j.mce.2016.10.006 PubMed 27720894
Related items: