FLAG-ARF
(Plasmid #89123)

• Purpose: mammalian expression of p14-hARF with N-terminal FLAG tag
• Depositing Lab: Elizabeth Wilson
• Publication: Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87. doi: 10.1074/jbc.M115.663641. Epub 2015 Sep 1.

Backbone

• Vector backbone: pCMV5 Flag b
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p14-hARF
• Alt name: human ARF
• Species: H. sapiens (human)
• Insert Size (bp): 546
• Entrez Gene: CDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry