pSG5-hAR(16)
(Plasmid
#89120)
-
Purposemammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSG5
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehAR
-
SpeciesH. sapiens (human)
-
Mutationinternal EcoRI site removed
-
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSG5-hAR(16) was a gift from Elizabeth Wilson (Addgene plasmid # 89120 ; http://n2t.net/addgene:89120 ; RRID:Addgene_89120) -
For your References section:
Domain interactions between coregulator ARA(70) and the androgen receptor (AR). Zhou ZX, He B, Hall SH, Wilson EM, French FS. Mol Endocrinol. 2002 Feb;16(2):287-300. doi: 10.1210/mend.16.2.0765. 10.1210/mend.16.2.0765 PubMed 11818501