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Addgene

pPS1307
(Plasmid #8912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HMT1
  • Alt name
    pRS316[HMT1-XbaI]
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2700
  • Entrez Gene
    HMT1 (a.k.a. YBR034C, HCP1, ODP1, RMT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2.7 kb XbaI genomic fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1307 was a gift from Pamela Silver (Addgene plasmid # 8912 ; http://n2t.net/addgene:8912 ; RRID:Addgene_8912)

  • For your References section:

    A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Henry MF, Silver PA. Mol Cell Biol. 1996 Jul . 16(7):3668-78. PubMed 8668183
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