pcDNA3myc-PR-LBD
(Plasmid
#89113)
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Purposemammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-myc
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman progesterone receptor ligand binding domain
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SpeciesH. sapiens (human)
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Mutationcontains Ligand Binding Domain (632-933)
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Entrez GenePGR (a.k.a. NR3C3, PR)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3myc-PR-LBD was a gift from Elizabeth Wilson (Addgene plasmid # 89113 ; http://n2t.net/addgene:89113 ; RRID:Addgene_89113) -
For your References section:
Gain in transcriptional activity by primate-specific coevolution of melanoma antigen-A11 and its interaction site in androgen receptor. Liu Q, Su S, Blackwelder AJ, Minges JT, Wilson EM. J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 10.1074/jbc.M111.244715. Epub 2011 Jul 5. 10.1074/jbc.M111.244715 PubMed 21730049