Skip to main content
Addgene

pPS1083
(Plasmid #8911)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPS892
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YRB2
  • Alt name
    pGAL:GST-YRB2
  • Alt name
    pYGEX(2T)-YRB2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1100
  • Entrez Gene
    YRB2 (a.k.a. YIL063C)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAL1 promoter, 2 micron origin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1083 was a gift from Pamela Silver (Addgene plasmid # 8911 ; http://n2t.net/addgene:8911 ; RRID:Addgene_8911)

  • For your References section:

    Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue. Taura T, Schlenstedt G, Silver PA. J Biol Chem. 1997 Dec 12. 272(50):31877-84. 10.1074/jbc.272.50.31877 PubMed 9395535
Related items: