pCMV-GR11
(Plasmid #89105)

• Purpose: mammalian expression of full-length human glucocorticoid receptor in pCMV5
• Depositing Lab: Elizabeth Wilson
• Publication: Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-27.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human glucocorticoid receptor
• Alt name: hGR
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: NR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results found a 4 AA insertion after G499 and an I747T mutation compared to NCBI reference sequence NP_000167.1. The depositor has confirmed that this construct produces functional GR.