GAL-p300-1000-1600
(Plasmid #89101)

• Purpose: mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain
• Depositing Lab: Elizabeth Wilson
• Publication: Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 10.1074/jbc.M110.120600. Epub 2010 May 6.

Backbone

• Vector backbone: pGALO
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: p300
• Species: H. sapiens (human)
• Mutation: aa 1000-1600
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Tag / Fusion Protein:
  • GAL4 DBD (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found a D1507N mutation in p300 compared to NCBI reference sequence NM_001429.3. This mutation is not known to affect plasmid function.