GAL-p300-230-670
(Plasmid
#89099)
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Purposemammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGALO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep300
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SpeciesH. sapiens (human)
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Mutationaa 230-670
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Entrez GeneEP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
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Tag
/ Fusion Protein
- GAL4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site ClaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL-p300-230-670 was a gift from Elizabeth Wilson (Addgene plasmid # 89099 ; http://n2t.net/addgene:89099 ; RRID:Addgene_89099) -
For your References section:
Transcriptional synergy between melanoma antigen gene protein-A11 (MAGE-11) and p300 in androgen receptor signaling. Askew EB, Bai S, Blackwelder AJ, Wilson EM. J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 10.1074/jbc.M110.120600. Epub 2010 May 6. 10.1074/jbc.M110.120600 PubMed 20448036
