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Addgene

VP-FKBP51
(Plasmid #89097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNLVP16
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FKBP5
  • Alt name
    FKBP51
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Entrez Gene
    FKBP5 (a.k.a. AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10)
  • Tags / Fusion Proteins
    • VP16 (N terminal on backbone)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VP-FKBP51 was a gift from Elizabeth Wilson (Addgene plasmid # 89097 ; http://n2t.net/addgene:89097 ; RRID:Addgene_89097)

  • For your References section:

    Primate-specific melanoma antigen-A11 regulates isoform-specific human progesterone receptor-B transactivation. Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, Wilson EM. J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 10.1074/jbc.M112.372797. Epub 2012 Aug 13. 10.1074/jbc.M112.372797 PubMed 22891251
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