pSG5-HA-p300-DY1399
(Plasmid #89095)

• Purpose: mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect
• Depositing Lab: Elizabeth Wilson
• Publication: Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 10.1074/jbc.M110.120600. Epub 2010 May 6.

Backbone

• Vector backbone: pSG5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p300-D1399Y mutant
• Species: H. sapiens (human)
• Insert Size (bp): 7800
• Mutation: p300-DY1399
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Promoter: SV40
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found a D1507N mutation in p300 compared to NCBI reference sequence NM_001429.3. This mutation is not known to affect plasmid function.