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Addgene

pAc-His-hAR-2
(Plasmid #89091)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcC4
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-His-hAR-2 was a gift from Elizabeth Wilson (Addgene plasmid # 89091 ; http://n2t.net/addgene:89091 ; RRID:Addgene_89091)

  • For your References section:

    Redox-dependent DNA binding of the purified androgen receptor: evidence for disulfide-linked androgen receptor dimers. Liao M, Zhou Zx, Wilson EM. Biochemistry. 1999 Jul 27;38(30):9718-27. doi: 10.1021/bi990589i. 10.1021/bi990589i PubMed 10423251
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