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pCMV-FLAG-MAGE-2-429
(Plasmid #89090)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5-FLAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAGE-A11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    MAGEA11 (a.k.a. CT1.11, MAGE-11, MAGE11, MAGEA-11)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-FLAG-MAGE-2-429 was a gift from Elizabeth Wilson (Addgene plasmid # 89090 ; http://n2t.net/addgene:89090 ; RRID:Addgene_89090)

  • For your References section:

    Melanoma antigen gene protein MAGE-11 regulates androgen receptor function by modulating the interdomain interaction. Bai S, He B, Wilson EM. Mol Cell Biol. 2005 Feb;25(4):1238-57. 10.1128/MCB.25.4.1238-1257.2005 PubMed 15684378
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