pCMV-hAR-L26A,F27A,W433A,L436A,F437A
(Plasmid #89085)

• Purpose: mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs
• Depositing Lab: Elizabeth Wilson
• Publication: He et al J Biol Chem. 2000 Jul 28;275(30):22986-94.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hAR FxxLF WxxLF mutant
• Species: H. sapiens (human)
• Mutation: hAR-L26A,F27A,W433A,L436A,F437A
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry