pET-15b-hAR-663-919
(Plasmid #89083)

• Purpose: bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag
• Depositing Lab: Elizabeth Wilson
• Publication: Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-16. doi: 10.1074/jbc.M703268200. Epub 2007 Jun 25.

Backbone

• Vector backbone: pET-15b
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hAR ligand binding domain
• Species: H. sapiens (human)
• Mutation: aa 663-919
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry