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Addgene

VP-hAR1-660
(Plasmid #89081)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNLVP16
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hAR-1-660
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-660 (contains exons A,B,C and part of D)
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter SV40
  • Tag / Fusion Protein
    • VP16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains a short VP16 activation domain (AA 411-456.)

Please also see https://www.ncbi.nlm.nih.gov/pubmed/10816582.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VP-hAR1-660 was a gift from Elizabeth Wilson (Addgene plasmid # 89081 ; http://n2t.net/addgene:89081 ; RRID:Addgene_89081)

  • For your References section:

    Intermolecular NH2-/carboxyl-terminal interactions in androgen receptor dimerization revealed by mutations that cause androgen insensitivity. Langley E, Kemppainen JA, Wilson EM. J Biol Chem. 1998 Jan 2;273(1):92-101. PubMed 9417052
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