pPS892
(Plasmid
#8908)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS293
- Backbone size (bp) 5200
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Alt namepYGEX-2T
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Yeast GST expression plasmid, XbaI oligo inserted into pGEX-2TB to convert XbaI site to BglII; 0.7-kb BglII-BamHI fragment of pGEX-2TB inserted into BamHI site of pPS293, GAL1 promoter, 2 micron origin. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS892 was a gift from Pamela Silver (Addgene plasmid # 8908 ; http://n2t.net/addgene:8908 ; RRID:Addgene_8908) -
For your References section:
Dynamic localization of the nuclear import receptor and its interactions with transport factors. Koepp DM, Wong DH, Corbett AH, Silver PA. J Cell Biol. 1996 Jun . 133(6):1163-76. 10.1083/jcb.133.6.1163 PubMed 8682856
Map uploaded by the depositor.
