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Addgene

pJK148-Pnmt41-GBP-mCherry(C)-leu1+
(Plasmid #89069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJK148
  • Vector type
    Yeast Expression ; genomic integration in S. pombe
  • Selectable markers
    leu1+
  • Tag / Fusion Protein
    • GBP-mCherry (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • GBP-mCherry (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results found a 1 bp deletion in leu1+ compared to the depositor sequence. This change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK148-Pnmt41-GBP-mCherry(C)-leu1+ was a gift from Quanwen Jin (Addgene plasmid # 89069 ; http://n2t.net/addgene:89069 ; RRID:Addgene_89069)

  • For your References section:

    Facile manipulation of protein localization in fission yeast through GBP-GFP binding. Chen YH, Wang GY, Hao HC, Chao CJ, Wang Y, Jin QW. J Cell Sci. 2017 Jan 12. pii: jcs.198457. doi: 10.1242/jcs.198457. 10.1242/jcs.198457 PubMed 28082423
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