-
Purpose(Empty Backbone) genomic GBP-mCherry epitope C-terminal tagging in S. pombe
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepFA6a
-
Vector typeYeast Expression ; PCR-based C-terminal tagging
-
Selectable markersNeomycin (select with G418)
-
Tag
/ Fusion Protein
- GBP-mCherry (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- GBP-mCherry (C terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's sequencing results found a G230M mutation in mCherry compared to the depositor sequence. This mutation is not known to affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a-GBP-mCherry-kanMX6 was a gift from Quanwen Jin (Addgene plasmid # 89068 ; http://n2t.net/addgene:89068 ; RRID:Addgene_89068) -
For your References section:
Facile manipulation of protein localization in fission yeast through GBP-GFP binding. Chen YH, Wang GY, Hao HC, Chao CJ, Wang Y, Jin QW. J Cell Sci. 2017 Jan 12. pii: jcs.198457. doi: 10.1242/jcs.198457. 10.1242/jcs.198457 PubMed 28082423
